zeynab ahmadi; hossein zolgharein; bita archangi; ebrahim rajabzade
Abstract
16S rRNA is one of the components of the ribosome and widely used in phylogenetic studies of closely related species and especially related species Crassostrea. Nucleotide sequence polymorphism associated with 16S rRNA gene of mitochondrial genome of Persian Gulf Oyster (Crassostrea sp.), examined in ...
Read More
16S rRNA is one of the components of the ribosome and widely used in phylogenetic studies of closely related species and especially related species Crassostrea. Nucleotide sequence polymorphism associated with 16S rRNA gene of mitochondrial genome of Persian Gulf Oyster (Crassostrea sp.), examined in 30 samples collected from Badar-e Emam Khomeini oysters. Through a primer PCR chain reaction was performed for all samples and product of PCR were Automated sequencing. Then the data obtained were analyzed by the programs and software. For the gene studied only there was 2 mutation and Nucleotide diversity (Pi) 0.00061 calculated. Among the 30 samples, 3 haplotypes were detected and average haplotype diversity (Hd) 0.195 were calculated. The haplotypes obtained in this study were first registered in the gene bank. Phylogeny trees drived from 16S rRNA gene sequencing showed no clear separation of the samples in the Bandar-e Imam Khomeini region. The results of this study showed that there is low sequence differentiation in the study population in Bandar-e Imam Khomeini.